[1] |
Fabrication of a novel bioactive chitosan based biocomposite from Opuntia ficus-indica fruit gum and evaluation of anticancer activity in bone MG63 cancer and L929 normal cell … G. Jaganathan In Inorganic Chemistry Communications, , Jan 2023, Volume: 147, Page: 110248.
Abstract [+]
In this paper, a novel bioactive Chitosan-Lignin/n-Hydroxyapatite/Opuntia ficus-indica -Fruit gum (Cs-Lg/n-Hap/OFI-Fg) biocomposite was fabricated. The as-obtained biocomposite properties are analyzed by optical and structural investigation. The biological properties like antibacterial, antioxidant, and anticancer ability were evaluated for the biocomposites. The FT-IR spectrum result shows the biocomposites possess significant intermolecular interaction with biomolecules. The powder XRD result indicated the as-obtained Cs-Lg/n-Hap/OFI-Fg biocomposites are crystalline in nature. The microhardness test shows the addition of fruit gum increases the mechanical strength of the biocomposite. The thermal stabilities of the as-obtained Cs-Lg/n-Hap/OFI-Fg biocomposite were higher than to Cs-Lg/n-Hap composite as revealed by TGA analysis. The SEM images show an addition of fruit gum leads to the formation of …
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[2] |
Novel beeswax-chitosan/Zinc-hydroxyapatite biocomposite porous scaffolds: Preparation and biological evaluation K. Manivannan In Journal of Science Advanced materials and devices , -, Jun 2021, Volume: 6, Page: 191.
Abstract [+]
We developed a novel BW-CS/ZnHA biocomposite utilizing Beeswax-Chitosan and Zinc-hydroxyapatite nanoparticles (ZnHAP). The uniformed mixture of beeswax-chitosan and ZnHAP was freeze-dried to get a porous biocomposite. The fabricated biocomposite was characterized using FTIR, XRD, and SEM. Moreover, the antibacterial, swelling, biodegradations, viability, and cell proliferation capacity of the fabricated biocomposite was assessed. The biocomposite demonstrated upgraded swelling and antibacterial activity. The addition of ZnHAP accomplished the antibacterial activity. Biocompatibility studies were carried out using osteoblast cells and thus we proved the non-toxic nature of the biocomposite. Osteoblast cell adhesion investigations demonstrated that the cells were appended and infiltrated into the inside of the biocomposite. The obtained results encourage the use of these bandages for various types of wounds with infection and exudate
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[3] |
Biologically active Chitosan/ZnO/Acalypha indica leaf extract biocomposite: An investigation of antibacterial, cell proliferation and cell migration aptitude for wound healing applications N. Sakthi guru In Sustainable Chemistry and Pharmacy, -, Apr 2021, Volume: 19, Page: 100357.
Abstract [+]
A biocomposite composed of Chitosan (Cs), Zinc oxide (ZnO), Acalypha indica (AI) was fabricated by simple chemical precipitation method. The achieved biocomposite was characterized by Fourier Transmission Infrared spectroscopy (FT-IR), X-ray diffraction spectroscopy (XRD), Scanning electron microscope (SEM) with Energy dispersive X-ray analysis spectroscopy (EDAX) and Transmission electron microscope (TEM). The phytochemical constituents in the AI leaf extract were also studied by Gas chromatography mass spectrometer (GC-MS) analysis. The anti-inflammatory and antioxidant studies were studied using Diclofenac sodium and ascorbic acid as standard by Fetal bovine serum denaturation and (2,2-diphenyl-1-picryl-hydrazyl-hydrate) DPPH radical scavenging method. The effective bactericidal action of the composite was evaluated against both gram-negative, gram-positive bacteria such as Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). The hemolysis activity was studied using Triton x 100 as negative and Phosphate buffer solution (PBS) as positive control. The biocompatibility nature was evaluated by the MTT (3- (4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT assay and Fluorescence staining method using mouse fibroblast cells (L929). Additionally, the in-vitro wound healing potential was also assessed by scratch wound assay with L929 mouse fibroblast cells. Hence, these obtained results suggest that the Cs/ZnO/AI biocomposite can act as a suitable candidate in wound care applications.
Reference1
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[4] |
Fabrication of bioinspired chitosan/gelatin/allantoin biocomposite film for wound dressing application Sakthiguru N In International journal of Biological Macromolecules , , Jun 2020, Volume: 152c, Page: 873-883.
Abstract [+]
A biocomposite film composed of biopolymers chitosan (CS), gelatin (GE) and allantoin (AT) was fabricated by solution casting technique. The functional group interaction of the biocomposite films was inspected through the Attenuated total reflectance Fourier transform spectrometer (ATR-FTIR). The morphological changes and crystallinity of biocomposite films with varied ratios of chitosan/gelatin (CS/GE) and allantoin were examined under Scanning electron microscope (SEM) and X-ray diffractometer (XRD). The water-absorbing capacity was found enhanced by an increase in the chitosan ratio. The biocomposite films exhibit good antioxidant, anti-inflammatory properties. The biocomposite films also display enhanced stability with steady degradation under the PBS medium. The biocomposite films reveal improved antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). Blood compatibility studies explore the non-hemolytic nature. The in vitro cytotoxicity by MTT assay shows excellent biocompatibility. The fibroblast adhesion on the biocomposite film displays enhanced proliferation and viability. These significant biological properties of biocomposite film make it an appropriate candidate for wound dressing application. Reference1
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[5] |
Preparation and In Vitro Biological Evaluation of Lawsone Loaded O-Carboxymethyl Chitosan/Zinc Oxide Nanocomposite for Wound-Healing Application N sakthi Guru In chemistry select , , Mar 2020, Volume: 5, Page: 2710.
Abstract [+]
A biopolymer based nanocomposite with O-Carboxymethyl
chitosan (O-Cmc), nano zinc oxide (ZnO) and Lawsone (Ln) with
proving advantages in antibacterial wound healing application
was prepared. The physiochemical characteristics of the nanocomposite
(NCs) was analysed by Fourier Transmission Infrared
spectroscopy (FT-IR), X-Ray Diffractometer (XRD), Scanning
electron Microscopy (SEM), Transmission electron microscope
(TEM) and Thermogravimetric analysis (TGA). The biological
properties such as anti-inflammatory, anti-oxidant activities
were investigated in vitro. The nanocomposite also possesses
efficient drug encapsulation and releasing profile with greater
stability in phosphate buffer saline (PBS) and Dulbecco’s
minimal essential medium (DMEM). The results of the antibacterial
studies show its enhanced bactericidal nature. The
results obtained from MTT assay, Ethidium bromide stained
fluorescence image and in vitro wound scratch assay show
greater cell viability with the closure of scratch wound
obviously proves it’s prominent biocompatible, wound closure
nature. These biological properties show that the prepared
nanocomposite has a greater potential to act as an excellent
biomaterial in the field of wound care applications.
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[6] |
Biologically Active Water Soluble Novel Biopolymer/Hydrazide Based OCarboxymethyl Chitosan Schiff Bases: Synthesis and Characterisation M. Manimohan In Journal of Inorganic and organometallic polymers and materials , , Feb 2020.
Abstract [+]
Six new N,N,O tridentate water soluble hydrazide based O-carboxymethyl chitosan Schiff base derivatives have been prepared
through in-situ synthesis. The degree of substitution of schiff base derivatives was calculated theoretically and the derivatives
were prepared with 1:1 proportion. Biopolymer Schiff base derivatives with their various bonding sites and different protoncarbon
environment were characterised by FT-IR and 1H, 13C NMR. The crystallinity of synthesized Schiff bases was studied
by X-ray diffraction. Thermal decomposition of all synthesized O-carboxymethyl chitosan Schiff base ligands was investigated
by thermo gravimetric analysis which revealed the presence of Schiff base functionality to elongate the temperature to > 300
ºC. Scanning electron microscopic studies revealed that O-carboxymethyl chitosan Schiff base derivatives were affecting the
crystallinity of chitosan. Additionally, the synthesized novel Schiff base derivatives were characterized for anti-inflammatory,
total antioxidant and antidiabetic studies and they showed better biological and pharmaceutical results with standard drug.
Graphic Abstract
Keywords Biopolymer schiff base derivatives · Carboxymethylation · Anti-inflammatory · Antidiabetic activity
Reference1
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[7] |
Zinc(II) centred biologically active novel N,N,O donor tridentate water – soluble hydrazide basd O- carboxy methane chitosan Schiff base metal complexes: synthesis and characterization, M. Manimohan In New Journal of Chemistry , , Jun 2019, Volume: 43, Page: 9540-9554.
Abstract [+]
In this study, novel eco-friendly and water-soluble chitosan Schiff base derivatives have been designed for potential use in antimicrobial applications. The novel water-soluble hydrazide-based O-carboxymethyl chitosan Schiff bases were prepared by carboxymethylation for potential applications in several biomedical and pharmaceutical avenues. Problems relating to the water solubility of hydrazide-based chitosan Schiff base derivatives have been eliminated. Thermogravimetric analysis (TGA) reveals the extended thermal stability of the complex even at an elevated temperature. The X-ray diffraction (XRD) studies reveal crystallinity that is destroyed after complexation. The scanning electron microscopy (SEM) images clearly reveal the crystalline and amorphous nature of the synthesized derivatives. The amorphous form finds wide applications in biological, pharmaceutical and biomedical fields. The synthesized water-soluble chitosan Schiff base derivatives and their metal complexes have been investigated for some biological and pharmacological applications and the studies have shown better antibacterial activity, antifungal assay, anti-inflammatory activity and total antioxidant nature of the complexes.
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[8] |
Biologically active novel N.N.O donor tridentate water soluble hydrazide based O- carboxymethyl chitosan Schiff base Cu(II) metal complexes: Synthesis and Characterization, M. Manimohan In International journal of Biological Macromolecule , , Jun 2019, Volume: 136, Page: 736 - 754.
Abstract [+]
Water soluble hydrazide based O-carboxymethyl chitosan Schiff base ligands have been prepared from chitosan and a mixture of benzoic acidhydrazide with β-diketone by carboxymethylation using mono chloroaceticacid (MCA) through in-situ reaction. The degree of deacetylation of chitosan was determined by elemental analysis and potentiometric titration. Schiff base ligands and the functional groups were characterised by FT-IR, 1H, and 13C NMR spectroscopic techniques. The crystallinity of ligands and complexes was evaluated by X-ray powder diffraction (XRD) studies. Thermal stability of all the synthesized compounds was investigated by thermo gravimetric analysis and differential thermal analysis (TGA-DTA) which revealed the presence of metal oxide (CuO) residue to elevate the temperature to >500 °C. Filed emission scanning electron microscopic (FESEM) analysis revealed that Cu (II) complexes were more amorphous in nature than chitosan and Schiff base ligands. Vibrating sample magnetometer (VSM) studies reported that the d9 configuration of the metal complex was paramagnetic in nature and the geometry of the complex was square pyramidal. The antimicrobial activity of the synthesized hydrazide Cu (II) complexes was characterised by agar plate method, anti-inflammatory study was characterised by egg albumin denaturation technique and total antioxidant activity was characterised by phosphomolybdenum method.
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[9] |
Synthesis and characterisation of water soluble bio macromolecules/ chemically modified phosphorylated chitosand and its derivatives y M Manimohan In International journal of science and Humanities, , Jun 2019, Volume: 5(1), Page: 45 - 54.
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[10] |
Fabrication and characterization nof Artocarpus heterophyllus waste derived lignin added chitosan bio composites for wound dressing application, Sustainable chemistry and Pharmacy ,10(2018), 27-32 G Jeganathan In Journal of Sustainable chemistry and Pharmacy, , Aug 2018, Volume: 10, Page: 27-32.
Abstract [+]
This paper discusses the preparation and characterization of biocomposite lignin (Lg) derived from Artocarpus
heterophyllus- chitosan (CS) (Lg-CS) for biomedical applications. To develop enhanced physico-chemical and
biological properties of CS was combined with the Lg at 1, 3, and 5 wt% concentrations. Fourier transform
Infrared spectroscopy (FT-IR) outcomes suggest the presence of hydrogen bonding between CS and Lg, scanning
electron microscopy (SEM) pictures reveal that Lg was uniformly dissolved in CS. As a result of effective interface
and uniform dispersion, the mechanical stability of CS has been significantly enhanced due to the presence of Lg.
The bio-compatibilities of the Lg-CS towards NIH 3t3 cells were also investigated in cell propagation analyzer
after day 3. The results of the above study reveal that the Lg-CS bio-composites bandages are found to transmit
the most convenient surrounding for the advancement of NIH 3t3 cells. The results suggest that the fabricated
bio-composites materials shall be suitable for applications as wound healing and dressing materials. Reference1
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[11] |
Novel Hybrid Chitosan Derivatives and Chalcones Moiety for some biological applications: Synthesis, Characterization , antimicrobial and anti cancer activity; E. Baggiaraj In Preprints, , Aug 2018, Volume: 1.
Abstract [+]
Versatile hybrid organic polymers are prepared using two active intermediates such as cynuric chloride and chitosan derivatives. The prepared chalcones are characterized by using FT-IR, UV, and proton NMR, thermal analysis and Minimum inhibitory Concentration. Thermal stability of the synthesized hybrid polymer is found using TGA and the hybrid chitosan derivative chalcone is thermally stable up to 270 °C. The antimicrobial activity of the prepared chitosan containing chalcone moiety are find out using Minimum Inhibitory Concentration (MIC) method. The synthesized versatile chalcone shows excellent antimicrobial activity against gram-negative bacteria such as Pseudomonas aeruginosa; and Gram-positive bacteria Chalcone containing halogen moiety shows high activity (MIC 7.8 µg/mL) than the hydroxyl containing chalcone. Cytotoxicity activity of the synthesized composites shows high activity.
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[12] |
Novel chalcones of 1-(4-(4-((6-((7 chloroqunoline – 4-yl) amino) hexyl amino) – 6 (( 4-nitrophenyl)amino)-1,3,5, triazin – 2-yl)amino)phenyl)ethanone for Biological applications: synthesis, characterization and Antimicrobial studies, Vol.7 Issue 2. ISSN : 2277-288X. 2017. E. Baggiaraj In Acta Chimica and Pharmaceutica Indica, , Apr 2017, Volume: 7(2).
Abstract [+]
A series of biologically active chalcones are prepared using 1-(4-(4-((6-((7chloroquinolin-4-yl)amino)hexyl)amino)-6-((4-
nitrophenyl)amino)-1,3,5-triazin-2-yl)amino)phenyl)ethanone and different substituted aromatic aldehyde under bio-friendly
method. Prepared chalcones are characterized by IR, 1H-NMR and tested for their antimicrobial activity against a gram positive
and gram negative bacteria's are using Minimum Inhibitory Concentration (MIC) method. Chlorine containing chalcones shows
high antibacterial activity against tested bacteria, among the synthesized chalcones.
Keywords: Bacteria; Chlorine; Chalcone; MIC; Antimicrobial activity
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[13] |
Novel Chalcones of 3-(4-(4-(4-Acetylphenoxyl)-6-(Nitrophenyl)amino)-1,3,5- Triazin-2yl)oxy)Phenyl)-1-(2,4-Dichlorophenyl)Prop-2-en-1one for Biological Applications: Synthesis , characterization and Antimicrobial Studies. E. Baggiaraj In Chem Xpress, , Jan 2017, Volume: 10 (1), Page: 116.
Abstract [+]
A series of bioactive chalcones are synthesized using 3-(4-(4-[4-acetylphenoxy]-6-([nitrophenyl]amino)-1,3,5-triazin-2-yl)oxy)phenyl)-1-(2,4-dichlorophenyl)prop-2-en-1-one (ACP) and different substituted aromatic aldehyde under solvent free conditions. Synthesized chalcones are characterized and tested for their antimicrobial activity against gram positive and gram negative bacteria’s. The synthesized chalcones are characterized by IR, 1H-NMR and UV-visible spectroscopic techniques. The antimicrobial activities of the chalcones are determined using minimum inhibitory concentration (MIC) method. Synthesized chalcones shows high activity against tested bacteria’s especially the chalcone containing chlorine moiety shows very high activity against tested gram positive bacteria Staphylococcus aureus at 7.81 μL/ml.
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[14] |
O-(2-hydroxy benzoyl ) chitosan/starch Blend; preparation characterization and antimicrobial activities, vol. 2 Issue 5, 46 – 50 ,2015 S. Jafar Sathik In International journal of chemical studies , , Feb 2015, Volume: 2(6), Page: 46 - 50.
Abstract [+]
bstract: Modification of Chitosan into its derivative is a convenient method to improve its properties like metalrnion adsorption, antimicrobial activity, etc. In this work the hydroxyl group of chitosan was modified byrnreacting with 2-hydroxybenzoic acid to obtain O-(2-hydroxybenzoyl) chitosan (OSCS). Further, thernprepared derivative was blended with starch in 1:1 ratio using glycerol as a film forming material. It wasrnnamed as OSCSB. The derivatives were characterised by FTIR, XRD and TGA to confirm theirrnformation. XRD and TGA results showed the decrease in crystalline nature and thermal stability of thernblend. The antibacterial activity was investigated against Escherichia coli (E.coli), Staphylococcusrnaureus (S.aureus) and Proteus mirabilis (P. mirabilis) by Zone of Inhibition method. The antifungalrnactivities against Candida albicans (C. albicans), Aspergillus niger (A. niger) and Aspergillus flavus (A.rnflavus) were also assessed by the same method. This study clearly showed that both ester derivatives andrnits blend having a significant effect against the tested microbes.
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[15] |
Synthesis and antimicrobial activity of salicyloyl chitosan and starch blend S. Jafar Sathik In International Journal of Phy. Chem and Biol. Sci. , , May 2014, Volume: Vol. 4, Page: 378 - 383.
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[16] |
Processing and characterisation of TiO2 composites from vegetable oil resources, Aboobucker Sithique In Journal of Polymer Engineering , , Aug 2011, Volume: 31, Page: 175.
Abstract [+]
TiO2 filled bio-based composites were prepared from epoxidized soy bean oil and Diglycidyl ether of bisphenol-A, in which the nano TiO2 particles were dispersed using ultra sonication method. Composites prepared with 1, 3, 5 and 7 weight percent level (wt%) of TiO2 particles were characterized for their thermal properties such as glass transition temperature and degradation stability. Thermo gravimetric analysis (TGA) showed that thermal stability of the composites increases up to 5 wt% addition of TiO2; thereafter it follows a decreasing trend, since the increased amount of nano sized particles makes it difficult for dispersion. The glass transition temperature of the composites was improved considerably. Dynamic mechanical analysis (DMA) further confirms the restricted chain mobility of the composites upon reinforcement. The significant improvements in mechanical properties such as tensile strength, tensile modulus, flexural strength, flexural modulus, and impact strength were achieved on reinforcement. The wide angle X-ray diffraction (WAXD) studies show that the nanoparticles are exfoliated in the soy based epoxy matrix system. The morphological behavior of composites studied using scanning electron microscopy (SEM) reveals the compatibility between the matrix and nanoparticles. The results showed that adding nano sized TiO2 particles can improve the properties of thermal, mechanical, compatibility and water resistance of these renewable resource based epoxy matrices suitable for engineering applications.
Keywords: DMA; nano TiO2 particles; soy based epoxy; TGA morphology; X-ray studies
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Preparation and characterization of zirconium oxide composites from vegetable oil resources, Aboobucker Sithique In Malaysian Polymer Journal, , Mar 2011, Volume: 6, Page: 1 - 13.
Abstract [+]
Bio-based nanocomposites were developed from epoxidized soy bean oil and diglycidyl ether of bisphenol-A, in which the nano sized zirconium oxide (ZrO2) particles were dispersed using ultra sonication method. Thermal stability of the nanocomposites were studied with thermo gravimetric analysis (TGA), restricted chain mobility and rigidity developed upon reinforcement results in reduced oxygen permeability leads to the enhanced thermal stability. The high storage modulus exhibited by dynamic mechanical analysis (DMA) showed that nanocomposites lead to rigidity upon reinforcement and it also confirms the restricted chain mobility of the composites. The structural morphological characteristics of the nanocomposites were investigated by wide angle X-ray diffraction (WAXD) analysis and scanning electron microscopy (SEM). The WAXD studies shows that the nanoparticles are exfoliated in the soy based epoxy matrix system. The SEM micrographs reveal the compatibility between the matrix and the nanoparticles without any aggregation. Significant improvements in mechanical properties such as tensile strength, tensile modulus, flexural strength, flexural modulus, impact strength of the composites were also reported.
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[18] |
Preparation And Properties Of Bio-Based Nanocomposites From Epoxidized Soy Bean Oil And Layered Silicate Aboobucker Sithique In Malaysian Polymer Journal, , Sep 2010, Volume: 5, Page: 151 - 161.
Abstract [+]
Bio-based nanocomposites have been developed from epoxidized soybean oil, diglycidyl ether of bisphenol-A (DGEBA) and organically modified montmorillonite (OMM). The organically modified montmorillonite and the nanocomposites were investigated by wide angle X-ray diffraction (WAXD) analysis and the morphology of these nanocomposites was examined by scanning electron microscopy (SEM). The exfoliated nanocomposites exhibited significant improvements in mechanical properties such as tensile strength, tensile modulus, flexural strength, flexural modulus and impact strength. Dynamic mechanical analysis (DMA) showed that nanocomposites exhibits high storage modulus and imparts rigidity upon reinforcement of the OMM. The nanocomposites were characterized for their thermal properties such as glass transition temperature, degradation stability and water absorption properties. There is almost a 24% increase in the tensile and flexural properties of the prepared nanocomposites upon reinforcement of 7wt% of the MMT. The restricted chain mobility and diffusion of oxygen and volatile products enhanced the thermal stability of the nanocomposites to a significant value.
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[19] |
Synthesis and Characterization of bismaleimide modified soy based epoxy matrices for flame retardant applications Aboobucker Sithique In High Performance Polymers , , Mar 2009, Volume: 22, Page: 328 - 344.
Abstract [+]
Epoxidized soybean oil at various concentrations was cured with amine curing agent. The prepared matrices were chemically modified with three types of bismaleimides, namely N,N′ -bismaleimido4,4 ′ -diphenylmethane (BMI-1), 1,3-bis(maleimido)benzene (BMI-2) and 3,3′ -bis(maleimidophenyl) phenylphosphine oxide (BMI-3). The interpenetrating networks thus developed were characterized for glass transition temperature, thermal stability, dynamic mechanical analysis, heat distortion temperature, flame retardancy and water absorption behavior. The incorporation of bismaleimides in the soy-based epoxy matrices significantly enhanced thermal stability and flame retardancy. The excellent flame retardant properties of the BMI-3 incorporated system were also demonstrated by high char yield and high limiting oxygen index. Dynamic mechanical analysis studies showed that the storage modulus increased with the increase in the concentration of bismaleimides and a reduction in the tan δ value for the bismaleimide-modified, 30 wt.% soy-based epoxy system due to enhanced cross-linking density and rigidity. Reference1
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[20] |
Mechanical and Morphological Properties of bismaleimide modified soy based epoxy matrices – Aboobucker Sithique In International Journal of Polymeric Materials, , , Mar 2008, Volume: 57, Page: 480-493.
Abstract [+]
This study is concerned with the preparation and mechanical characterization of bio-based polymers from renewable resources. Epoxidized soybean oil at various concentrations is cured with an amine curing agent. The prepared matrices have been chemically modified with three types of bismaleimides, namely N, N′-bismaleimido-4, 4′-diphenyl methane (BMI-1), 1,3-bis(maleimido)benzene(BMI-2) and 3,3′-bis(maleimido phenyl)phenyl phosphineoxide (BMI-3). The crosslinked matrices thus developed were characterized for their mechanical properties such as tensile strength, tensile modulus, flexural strength, flexural modulus, and impact strength. The incorporation of bismaleimides in the soy-based matrices significantly enhances the mechanical properties. The morphological behavior of matrices is also studied using a scanning electron microscope. The results indicate that the bismaleimide-modified soy-based epoxy resin at appropriate concentration holds great potential as a replacement for petroleum-based materials in engineering applications.
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